WB
Various lysates were subjected to SDS PAGE followed by western blot with TGN46 antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using TGN46 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
IF result of TGN46 antibody (1:50) with Human Fibroblast (primary cells) By Dr. Neeraj Tiwar, Rothman Lab, Yale School of Medicine. Fixed with 4% PFA 10 min.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed human cerebellum tissue using TGN46 antibody, at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-TGN46 ( 3ug; 1:300) with HeLa cells lysate 2400ug.| Product Name | TGN46 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TGOLN2,TGN46 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:5000-1:50000 IHC:1:400-1:1600 ICC/IF:1:1000-1:4000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 0.3mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TGOLN2 |
|---|---|
| Gene Synonyms | TGN38 TGN46 TGN48 TGN51 TTGN2 hTGN46 hTGN48 hTGN51 |
| Gene Full Name | trans-golgi network protein 2 |
| Gene Summary | This gene encodes a type I integral membrane protein that is localized to the trans-Golgi network, a major sorting station for secretory and membrane proteins. The encoded protein cycles between early endosomes and the trans-Golgi network, and may play a role in exocytic vesicle formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011] |
| Molecular Weight(MW) | 47 kDa |
WB
Various lysates were subjected to SDS PAGE followed by western blot with TGN46 antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using TGN46 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
IF result of TGN46 antibody (1:50) with Human Fibroblast (primary cells) By Dr. Neeraj Tiwar, Rothman Lab, Yale School of Medicine. Fixed with 4% PFA 10 min.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed human cerebellum tissue using TGN46 antibody, at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-TGN46 ( 3ug; 1:300) with HeLa cells lysate 2400ug.| Application Notes | WB:1:5000-1:50000 IHC:1:400-1:1600 ICC/IF:1:1000-1:4000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
