WB
Western blot analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: human A549 whole cell lysates, Lane 8: human HL-60 whole cell lysates, Lane 9: rat lung tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Galectin-3/LGALS3 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for Galectin-3/LGALS3 at approximately 29 kDa. The expected band size for Galectin-3/LGALS3 is at 26 kDa.IHC
IHC analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody. Galectin-3/LGALS3 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Galectin-3/LGALS3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody. Galectin-3/LGALS3 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-Galectin-3/LGALS3 Antibody (M00621-3) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of U2OS cells using anti-Galectin-3/LGALS3 antibody. Overlay histogram showing U2OS cells stained with M00621-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Galectin-3/LGALS3 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-Galectin-3/LGALS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A/G |
| Gene Symbol | LGALS3 |
|---|---|
| Gene Synonyms | L31 GAL3 MAC2 CBP35 GALBP GALIG LGALS2 |
| Gene Full Name | galectin 3 |
| Gene Summary | This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014] |
| Alternative Names | 35 kDa lectin CBP 35 CBP35 Gal 3 GAL3 Galactose specific lectin 3 Galactoside binding protein GALBP Galectin 3 Galectin-3 GALIG IgE binding protein L 31 Laminin binding protein Lectin L 29 LGALS2 LGALS3 Mac 2 antigen MAC2 |
| Molecular Weight(MW) | 26kDa(Observed MW 29kDa) |
| Cellular Localization | Cytoplasm,Nucleus,Secreted,Spliceosome |
WB
Western blot analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: human A549 whole cell lysates, Lane 8: human HL-60 whole cell lysates, Lane 9: rat lung tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Galectin-3/LGALS3 antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for Galectin-3/LGALS3 at approximately 29 kDa. The expected band size for Galectin-3/LGALS3 is at 26 kDa.
IHC
IHC analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody. Galectin-3/LGALS3 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Galectin-3/LGALS3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody. Galectin-3/LGALS3 was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-Galectin-3/LGALS3 Antibody (M00621-3) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of U2OS cells using anti-Galectin-3/LGALS3 antibody. Overlay histogram showing U2OS cells stained with M00621-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Galectin-3/LGALS3 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-mouse IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
