WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: PC-12 Lane2: 3T3-L1 Lane3: PC-3 Lane4: U-14 Predicted band size: 15kDa Observed band size: 15kDaWB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Citrulline Arg2) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 3 hours Predicted band size: 15kDa Observed band size: 17kDaWB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Citrulline Arg2) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HEK293 transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours Predicted band size: 15kDa Observed band size: 15kDa| Product Name | Histone H3 (Citrulline Arg2) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB1:1000-1:5000 IF1:200-1:1000 ELISA1:5000-1:20000 IP1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | H2BC15 |
|---|---|
| Gene Synonyms | H2B/d H2BFD HIST1H2BN |
| Gene Full Name | H2B clustered histone 15 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | HIST1H3A H3FA HIST1H3B H3FL HIST1H3C H3FC HIST1H3D H3FB HIST1H3E H3FD HIST1H3F H3FI HIST1H3G H3FH HIST1H3H H3FK HIST1H3I H3FF HIST1H3J H3FJ Histone H3.1 Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l HIST2H3A HIST2H3C H3F2 H3FM HIST2H3D Histone H3.2 Histone H3/m Histone H3/o H3F3A H3.3A H3F3 PP781 H3F3B H3.3B Histone H3.3 |
| Molecular Weight(MW) | 15 kDa |
| Cellular Localization | Chromosome,Nucleosome core,Nucleus |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: PC-12 Lane2: 3T3-L1 Lane3: PC-3 Lane4: U-14 Predicted band size: 15kDa Observed band size: 15kDa
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Citrulline Arg2) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 3 hours Predicted band size: 15kDa Observed band size: 17kDa
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Citrulline Arg2) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HEK293 transfected with PADI4 treated with 10 mM calcium chloride and 10 μM lonomycin for 4 hours Predicted band size: 15kDa Observed band size: 15kDa| Application Notes | WB1:1000-1:5000 IF1:200-1:1000 ELISA1:5000-1:20000 IP1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
