WB
Western blot analysis of ATP7A using anti-ATP7A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat liver tissue lysates, Lane 2: SMMC tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATP7A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for ATP7A at approximately 163 kDa. The expected band size for ATP7A is at 163 kDa.IHC
IHC analysis of ATP7A using anti-ATP7A antibody. ATP7A was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP7A Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Product Name | Anti-ATP7A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-2000 IHC:1:50-400 |
|
| Species Reactivity | HumanMouseRabbitRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASS1P9 |
|---|---|
| Gene Synonyms | ASSP9 |
| Gene Full Name | argininosuccinate synthetase 1 pseudogene 9 |
| Alternative Names | Copper pump 1 DSMAX MK MNK SMAX3 ATP7A MC1 |
| Molecular Weight(MW) | 163 kDa |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasm,Endoplasmic reticulum,Endosome |
WB
Western blot analysis of ATP7A using anti-ATP7A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Rat liver tissue lysates, Lane 2: SMMC tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATP7A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for ATP7A at approximately 163 kDa. The expected band size for ATP7A is at 163 kDa.
IHC
IHC analysis of ATP7A using anti-ATP7A antibody. ATP7A was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP7A Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.| Application Notes | WB:1:500-2000 IHC:1:50-400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
