WB
Western blot analysis of MEIOB using anti-MEIOB antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MEIOB antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for MEIOB at approximately 49 kDa. The expected band size for MEIOB is at 49 kDa.ICC/IF
ICC/IF analysis of MEIOB using anti-MEIOB antibody. MEIOB was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-MEIOB Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of K562 cells using anti-MEIOB antibody. Overlay histogram showing K562 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEIOB Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-MEIOB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human MEIOB recombinant protein (Position: I140-V442). Human MEIOB shares 77.9% and 76.1% amino acid (aa) sequence identity with mouse and rat MEIOB, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCMICC/IFWB |
| WB:1:500-2000 ICC/IF:1:50-1:400 FC:1:50-200 ELISA:1:100-1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MEIOB |
|---|---|
| Gene Synonyms | POF23 gs129 SPGF22 C16orf73 |
| Gene Full Name | meiosis specific with OB-fold |
| Gene Summary | Predicted to enable chromatin binding activity; single-stranded DNA 3'-5' DNA exonuclease activity; and single-stranded DNA binding activity. Predicted to be involved in double-strand break repair via homologous recombination; fertilization; and meiotic nuclear division. Located in nucleus. Implicated in primary ovarian insufficiency and spermatogenic failure 22. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | MEIOB C16orf73 Meiosis-specific with OB domain-containing protein EC 3.1.-.- |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Chromosome,Cytoplasm,Nucleus |
WB
Western blot analysis of MEIOB using anti-MEIOB antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MEIOB antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for MEIOB at approximately 49 kDa. The expected band size for MEIOB is at 49 kDa.
ICC/IF
ICC/IF analysis of MEIOB using anti-MEIOB antibody. MEIOB was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-MEIOB Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of K562 cells using anti-MEIOB antibody. Overlay histogram showing K562 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEIOB Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 ICC/IF:1:50-1:400 FC:1:50-200 ELISA:1:100-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
