WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ATR (Phospho Ser428) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela treated with UV for 1 hour, then recovered for 1 hour Predicted band size: 301kDa Observed band size: 290kDa| Product Name | ATR (Phospho Ser428) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| IHC:1:200-1:1000 WB:1:500-1:2000IF:1:200-1:1000ELISA:1:5000-1:20000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATR |
|---|---|
| Gene Synonyms | FRP1 MEC1 SCKL FCTCS SCKL1 |
| Gene Full Name | ATR checkpoint kinase |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1. [provided by RefSeq, Aug 2017] |
| Alternative Names | ATR FRP1 Serine/threonine-protein kinase ATR Ataxia telangiectasia and Rad3-related protein FRAP-related protein 1 |
| Molecular Weight(MW) | 301kD(Observed band size:290kD) |
| Cellular Localization | Chromosome,Nucleus |
WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ATR (Phospho Ser428) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela treated with UV for 1 hour, then recovered for 1 hour Predicted band size: 301kDa Observed band size: 290kDa| Application Notes | IHC:1:200-1:1000 WB:1:500-1:2000IF:1:200-1:1000ELISA:1:5000-1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
