WB
Western blot analysis of GABRG2 using anti-GABRG2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GABRG2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GABRG2 at approximately 54 kDa. The expected band size for GABRG2 is at 54 kDa.IHC
IHC analysis of GABRG2 using anti-GABRG2 antibody. GABRG2 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GABRG2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of THP-1 cells using anti-GABRG2 antibody. Overlay histogram showing THP-1 cells stained with A02361-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GABRG2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-GABRG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GABA A Receptor gamma 2/GABRG2 recombinant protein (Position: Q40-R446). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCIHCWB |
| WB:1:500-2000 IHC:1:50-400 ELISA:1:100-1000 FC:1:50-200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GABRG2 |
|---|---|
| Gene Synonyms | CAE2 ECA2 FEB8 DEE74 EIEE74 GEFSP3 |
| Gene Full Name | gamma-aminobutyric acid type A receptor subunit gamma2 |
| Gene Summary | This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | CAE2 ECA2 GABA A receptor gamma 2 GABAA receptor gamma 2 GABRG2 GEFSP3 |
| Molecular Weight(MW) | 54 kDa |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasmic vesicle,Membrane,Postsynaptic cell membrane,Synapse |
WB
Western blot analysis of GABRG2 using anti-GABRG2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GABRG2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GABRG2 at approximately 54 kDa. The expected band size for GABRG2 is at 54 kDa.
IHC
IHC analysis of GABRG2 using anti-GABRG2 antibody. GABRG2 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-GABRG2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of THP-1 cells using anti-GABRG2 antibody. Overlay histogram showing THP-1 cells stained with A02361-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GABRG2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 ELISA:1:100-1000 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
