WB
Western blot analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SPG3A/ATL1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for SPG3A/ATL1 at approximately 64 kDa. The expected band size for SPG3A/ATL1 is at 64 kDa.ICC/IF
ICC/IF analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody. SPG3A/ATL1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SPG3A/ATL1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of SH-SY5Y cells using anti-SPG3A/ATL1 antibody. Overlay histogram showing SH-SY5Y cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPG3A/ATL1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Anti-SPG3A/ATL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human RENT1/hUPF1 identical to the related mouse and rat sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCICC/IFWB |
| WB:1:500-2000 ELISA:1:100-1000 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATL1 |
|---|---|
| Gene Synonyms | FSP1 SPG3 ATL-1 HSN1D SPG3A AD-FSP atlastin1 |
| Gene Full Name | atlastin GTPase 1 |
| Gene Summary | The protein encoded by this gene is a GTPase and a Golgi body transmembrane protein. The encoded protein can form a homotetramer and has been shown to interact with spastin and with mitogen-activated protein kinase kinase kinase kinase 4. This protein may be involved in axonal maintenance as evidenced by the fact that defects in this gene are a cause of spastic paraplegia type 3. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | ATL1 GBP3 SPG3A Atlastin-1 Brain-specific GTP-binding protein GTP-binding protein 3 GBP-3 hGBP3 Guanine nucleotide-binding protein 3 Spastic paraplegia 3 protein A |
| Molecular Weight(MW) | 64 kDa |
| Cellular Localization | Cell projection,Endoplasmic reticulum,Golgi apparatus,Membrane |
WB
Western blot analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SPG3A/ATL1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for SPG3A/ATL1 at approximately 64 kDa. The expected band size for SPG3A/ATL1 is at 64 kDa.
ICC/IF
ICC/IF analysis of SPG3A/ATL1 using anti-SPG3A/ATL1 antibody. SPG3A/ATL1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-SPG3A/ATL1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of SH-SY5Y cells using anti-SPG3A/ATL1 antibody. Overlay histogram showing SH-SY5Y cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPG3A/ATL1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-2000 ELISA:1:100-1000 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
