WB
Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg) All lanes: TMEM176B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilutionICC/IF
Immunofluorescence staining of Hela cell with TMEM176B Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).IHC
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | TMEM176B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from human TMEM176B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 IF:1:10-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | TMEM176B |
|---|---|
| Gene Synonyms | LR8 MS4B2 |
| Gene Full Name | transmembrane protein 176B |
| Gene Summary | Predicted to be involved in negative regulation of dendritic cell differentiation. Predicted to be located in nuclear membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | TMEM176B LR8 Transmembrane protein 176B Protein LR8 |
| Molecular Weight(MW) | 30,26 kDa （Observed band size: 30 kDa） |
| Cellular Localization | Nucleus membrane; Multi-pass membrane protein. |
WB
Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg) All lanes: TMEM176B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution
ICC/IF
Immunofluorescence staining of Hela cell with TMEM176B Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IF:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
