WB
Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.| Product Name | SGK1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SGK around the phosphorylation site of Ser422. AA range:381-430 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SGK1 |
|---|---|
| Gene Synonyms | SGK |
| Gene Full Name | serum/glucocorticoid regulated kinase 1 |
| Gene Summary | This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009] |
| Alternative Names | SGK1 SGK Serine/threonine-protein kinase Sgk1 Serum/glucocorticoid-regulated kinase 1 |
| Molecular Weight(MW) | 57kD |
| Cellular Localization | Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cell membrane. Mitochondrion. The subcellular localization is controlled by the cell cycle , as well as by exposure to specific hormones and environmental stress stimuli. In proliferating cells , it shuttles between the nucleus and cytoplasm in synchrony with the cell cycle , and in serum/growth factor-stimulated cells it resides in the nucleus. In contrast , after exposure to environmental stress or treatment with glucocorticoids , it is detected in the cytoplasm and with certain stress conditions is associated with the mitochondria. In osmoregulation through the epithelial sodium channel , it can be localized to the cytoplasmic surface of the cell membrane. Nuclear , upon phosphorylation.; [Isoform 2]: Cell membrane. |
WB
Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
