WB
Western blot analysis of Glutathione Peroxidase 2 on different lysates with Rabbit anti-Glutathione Peroxidase 2 antibody at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: LoVo cell lysate Lane 3: HT-29 cell lysate Lane 4: AGS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | Glutathione Peroxidase 2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within C terminal Human Glutathione Peroxidase 2/GPX2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1,000-1:2,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GPX2 |
|---|---|
| Gene Synonyms | GPRP GPx-2 GI-GPx GPRP-2 GPx-GI GSHPx-2 GSHPX-GI |
| Gene Full Name | glutathione peroxidase 2 |
| Gene Summary | The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is predominantly expressed in the gastrointestinal tract (also in liver in human), is localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. Overexpression of this gene is associated with increased differentiation and proliferation in colorectal cancer. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016] |
| Alternative Names | Gastrointestinal glutathione peroxidase antibody GI GPx antibody Glutathione peroxidase 2 (gastrointestinal) antibody Glutathione peroxidase 2 antibody Glutathione peroxidase gastrointestinal antibody Glutathione peroxidase related protein 2 antibody Glutathione peroxidase-gastrointestinal antibody Glutathione peroxidase-related protein 2 antibody GPRP antibody GPRP-2 antibody Gastrointestinal glutathione peroxidase antibody GI GPx antibody Glutathione peroxidase 2 (gastrointestinal) antibody Glutathione peroxidase 2 antibody Glutathione peroxidase gastrointestinal antibody Glutathione peroxidase related protein 2 antibody Glutathione peroxidase-gastrointestinal antibody Glutathione peroxidase-related protein 2 antibody GPRP antibody GPRP-2 antibody GPx 2 antibody GPx-2 antibody GPx-GI antibody GPX2 antibody GPX2_HUMAN antibody GSHPx 2 antibody GSHPx GI antibody GSHPx-2 antibody GSHPx-GI antibody |
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytoplasm |
WB
Western blot analysis of Glutathione Peroxidase 2 on different lysates with Rabbit anti-Glutathione Peroxidase 2 antibody at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: LoVo cell lysate Lane 3: HT-29 cell lysate Lane 4: AGS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1,000-1:2,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
