WB
Western blot analysis of TMEM25 expression in OVCAR3 (A), mouse kidney (B), rat brain (C) whole cell lysates. (Predicted band size: 23; 33; 34; 37; 39 kD; Observed band size: 50 kD)ICC/IF
Immunofluorescent analysis of TMEM25 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Product Name | Anti-TMEM25 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human TMEM25. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TMEM25 |
|---|---|
| Gene Full Name | transmembrane protein 25 |
| Gene Summary | Predicted to be involved in negative regulation of excitatory postsynaptic potential and regulation of protein stability. Predicted to be located in late endosome and lysosome. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Transmembrane protein 25 |
| Molecular Weight(MW) | Predicted band size: 23; 33; 34; 37; 39 kD; (Observed band size: 50 kD) |
WB
Western blot analysis of TMEM25 expression in OVCAR3 (A), mouse kidney (B), rat brain (C) whole cell lysates. (Predicted band size: 23; 33; 34; 37; 39 kD; Observed band size: 50 kD)
ICC/IF
Immunofluorescent analysis of TMEM25 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
