WB
Western blot analysis of SEC61A on different lysates with Rabbit anti-SEC61A antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (hot lysis) Lane 2: HeLa cell lysate (no heat) Lane 3: A431 cell lysate (no heat) Lane 4: U-87 MG cell lysate (no heat) Lane 5: NIH/3T3 cell lysate (no heat) Lane 6: C6 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 45 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SEC61A antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | SEC61A Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human SEC61A2 aa 327-476 / 476. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GYG1 |
|---|---|
| Gene Synonyms | GYG GSD15 |
| Gene Full Name | glycogenin 1 |
| Gene Summary | This gene encodes a member of the glycogenin family. Glycogenin is a glycosyltransferase that catalyzes the formation of a short glucose polymer from uridine diphosphate glucose in an autoglucosylation reaction. This reaction is followed by elongation and branching of the polymer, catalyzed by glycogen synthase and branching enzyme, to form glycogen. This gene is expressed in muscle and other tissues. Mutations in this gene result in glycogen storage disease XV. This gene has pseudogenes on chromosomes 1, 8 and 13 respectively. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010] |
| Alternative Names | chunp6898 antibody fb62c11 antibody HSEC61 antibody protein transport protein SEC61 alpha subunit antibody protein transport protein Sec61 subunit alpha antibody Protein transport protein Sec61 subunit alpha isoform 1 antibody S61A1_HUMAN antibody SEC 61 antibody SEC 61A antibody Sec61 alpha 1 antibody chunp6898 antibody fb62c11 antibody HSEC61 antibody protein transport protein SEC61 alpha subunit antibody protein transport protein Sec61 subunit alpha antibody Protein transport protein Sec61 subunit alpha isoform 1 antibody S61A1_HUMAN antibody SEC 61 antibody SEC 61A antibody Sec61 alpha 1 antibody Sec61 alpha 1 subunit antibody Sec61 alpha 1 subunit (S. cerevisiae) antibody Sec61 alpha-1 antibody SEC61 antibody Sec61 homolog antibody SEC61A1 antibody sec61aa antibody |
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane |
WB
Western blot analysis of SEC61A on different lysates with Rabbit anti-SEC61A antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (hot lysis) Lane 2: HeLa cell lysate (no heat) Lane 3: A431 cell lysate (no heat) Lane 4: U-87 MG cell lysate (no heat) Lane 5: NIH/3T3 cell lysate (no heat) Lane 6: C6 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 45 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SEC61A antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
