| Species and genus |
Dengue virus 2 Thailand/16681/84 |
| Expression System |
HEK 293 cells |
| Amino acid sequence |
776 to 1127 |
| Purity |
>95% SDS-PAGE |
| Activity |
SDS-PAGE |
| Conjugate |
His tag C-Terminus |
| Form |
Liquid |
| Inclusion |
pH: 7-8,PBS. |
| Save conditions |
-80°C ,Avoid freeze / thaw cycle |
| Transportation conditions |
Dry Ice |
| Target information |
This supplementary information is collated from multiple sources and compiled automatically.
The Dengue virus 2 NS1 often referred to as DenV NS1 or NS1 protein dengue plays a critical role in the viral lifecycle and pathogenicity. This non-structural protein with a mass of approximately 46 kDa is expressed both intracellularly and extracellularly in host cells. Inside the cell NS1 localizes mainly in the endoplasmic reticulum while extracellular NS1 circulates in the bloodstream. The protein is known for its multifunctional properties acting as a molecular chaperone during viral replication and influencing host immune system responses.
Biological function summary
The NS1 protein impacts several cellular processes within the host. NS1 is not part of a larger protein complex but functions significantly on its own to facilitate a favorable environment for the virus. It assists in immune evasion by modulating host immune signaling pathways and inhibiting complement activation. NS1 can also bind to and disrupt endothelial cells which contributes to vascular leakage a severe clinical symptom associated with dengue virus infection.
Pathways
NS1 protein interacts with several pathways in its pursuit of viral propagation. It engages notably with the unfolded protein response (UPR) and the Toll-like receptor (TLR) pathways. Within the UPR NS1 modulates stress responses to maintain cellular homeostasis under viral replication conditions. In the context of the TLR pathway NS1 is recognized by TLR4 leading to the production of inflammatory cytokines. NS1's interactions within these pathways are important for understanding how dengue virus evades host immune responses.
Dengue virus 2 NS1 is closely associated with the pathology of dengue fever and dengue hemorrhagic fever. The protein contributes to disease severity through its ability to disrupt endothelial integrity and trigger immune system dysregulation. NS1's interaction with other non-structural proteins such as NS3 exacerbates these effects promoting the virus's ability to replicate efficiently and cause systemic inflammation. Understanding the role of NS1 in these conditions is essential for developing targeted therapies and vaccines against dengue virus infections. |
