WB
Western blot analysis of anti- FGB antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse liver tissue lysates. Use rabbit anti- FGB 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit . A specific band was detected for FGB at approximately 56KD. The expected band size for FGB is at 56KD.IHC
IHC analysis of Fibrinogen Beta Chain/FGB using anti-Fibrinogen Beta Chain/FGB antibody. Fibrinogen Beta Chain/FGB was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Fibrinogen Beta Chain/FGB Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of Fibrinogen Beta Chain/FGB using anti-Fibrinogen Beta Chain/FGB antibody. Fibrinogen Beta Chain/FGB was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Fibrinogen Beta Chain/FGB Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-Fibrinogen Beta Chain/FGB antibody. Overlay histogram showing HepG2 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibrinogen Beta Chain/FGB Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-Fibrinogen Beta Chain/FGB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human FGB, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FGB |
|---|---|
| Gene Synonyms | HEL-S-78p |
| Gene Full Name | fibrinogen beta chain |
| Gene Summary | The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Fibrinogen serves key roles in hemostasis and antimicrobial host defense. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. [provided by RefSeq, Aug 2020] |
| Alternative Names | FGB fibrinogen beta chain |
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of anti- FGB antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse liver tissue lysates. Use rabbit anti- FGB 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit . A specific band was detected for FGB at approximately 56KD. The expected band size for FGB is at 56KD.
IHC
IHC analysis of Fibrinogen Beta Chain/FGB using anti-Fibrinogen Beta Chain/FGB antibody. Fibrinogen Beta Chain/FGB was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Fibrinogen Beta Chain/FGB Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of Fibrinogen Beta Chain/FGB using anti-Fibrinogen Beta Chain/FGB antibody. Fibrinogen Beta Chain/FGB was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-Fibrinogen Beta Chain/FGB Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI(Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-Fibrinogen Beta Chain/FGB antibody. Overlay histogram showing HepG2 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fibrinogen Beta Chain/FGB Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
