WB
Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4℃ over nightIF
Immunofluorescence analysis of Hela cell. 1,MARCH5 Polyclonal Antibody (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.| Product Name | MYPT1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MARCH5. AA range:21-70 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| WB:1:500-1:2000 IF:200-1:1000 ELISA:1:40000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MARCHF5 |
|---|---|
| Gene Synonyms | MITOL MARCH5 RNF153 MARCH-V |
| Gene Full Name | membrane associated ring-CH-type finger 5 |
| Gene Summary | MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008] |
| Alternative Names | MARCH5 RNF153 E3 ubiquitin-protein ligase MARCH5 Membrane-associated RING finger protein 5 Membrane-associated RING-CH protein V MARCH-V Mitochondrial ubiquitin ligase MITOL RING finger protein 153 |
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane,Mitochondrion,Mitochondrion outer membrane,Peroxisome |
WB
Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4℃ over nightIF
Immunofluorescence analysis of Hela cell. 1,MARCH5 Polyclonal Antibody (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.| Application Notes | WB:1:500-1:2000 IF:200-1:1000 ELISA:1:40000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
