| Product Name | Mouse Fc Receptor Blocking Solution (CD16/CD32) Rat Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | Ig2b |
| Host Species | Rat |
| Tested Applications | FC |
| FC:1:50-1:200 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AI528646 CD16 CD32 F630109E10Rik Fc gamma receptor IIB Fc gamma receptor III Fc gamma RIIB Fc receptor, IgG, low affinity IIb Fc receptor, IgG, low affinity III Fc-gamma RII Fc-gamma RIII Fc-gamma-RII Fc-gamma-RIIB Fc[g] Fc[g]RII Fcg Fcg receptor III Fcgam Fcgamm FcgammaRIIB FcgammaRIII Fcgr Fcgr2 Fcgr2a FCGR2A Fcgr2b FCGR2B iso2 Fcgr3 FcgRII Fcr- Fcr-2 Fcr-3 FcRII FcRIII IgG Fc receptor II beta IgG Fc receptor III Low affinity immunoglobulin gamma Fc region receptor II low affinity immunoglobulin gamma Fc region receptor II-b Low affinity immunoglobulin gamma Fc region receptor III Ly-1 Ly-17 Ly-m2 Ly-m20 LyM- LyM-1 Lymphocyte antigen 17 OTTMUSP00000024374 OTTMUSP00000024375 |
|---|---|
| Gene Summary | The 2.4G2 clone is widely used as a blocking reagent to prevent interactions between mouse Fc receptors and IgG. By binding to CD32 (FcgammaRII) and CD16 (FcgammaRIII), the 2.4G2 clone prevents the Fc receptor proteins from being able to interact with the Fc domain of antibodies subsequently added for analysis. Performing an Fc blocking step thus reduces background and false positive events in flow cytometry assays on live cells. |
| Application Notes | FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Do not freeze. |
| Storage Buffer | Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH7.2 ,40% Glycerol. |
