WB
Western blot analysis of CD32A + CD32B + CD32C on different lysates with Rabbit anti-CD32A + CD32B + CD32C antibody at 1/1,000 dilution. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-CD32A + CD32B + CD32C antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | CD32A + CD32B + CD32C Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human CD32A aa 60-109 / 317. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:500-1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FCGR2A |
|---|---|
| Gene Synonyms | CD32 FCG2 FcGR CD32A CDw32 FCGR2 IGFR2 FCGR2A1 FcgammaRIIa |
| Gene Full Name | Fc gamma receptor IIa |
| Gene Summary | This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008] |
| Alternative Names | Low affinity immunoglobulin gamma Fc region receptor II-a Low affinity immunoglobulin gamma Fc region receptor II-b Low affinity immunoglobulin gamma Fc region receptor II-c IgG Fc receptor II-a IgG Fc receptor II-b IgG Fc receptor II-c CDw32 Fc-gamma RII-a (Fc-gamma-RIIa FcRII-a) Fc-gamma RII-b (Fc-gamma-RIIb FcRII-b) Fc-gamma RII-c (Fc-gamma-RIIc FcRII-c) CD32 FCGR2A FCGR2B FCGR2C CD32 FCG2 FCGR2A1 IGFR2 |
| Molecular Weight(MW) | 35 kDa(Observed band size:35-40 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD32A + CD32B + CD32C on different lysates with Rabbit anti-CD32A + CD32B + CD32C antibody at 1/1,000 dilution. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-CD32A + CD32B + CD32C antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
