WB
Western blot analysis of CD32 on different lysates with Rabbit anti-CD32 antibody at 1/2,000 dilution. Lane 1: THP-1 cell lysate, Lane 2: THP-1 cell lysate treated with deglycosylation, Lane 3: K-562 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD32 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of K-562 cells labeling CD32 with Rabbit anti-CD32 antibody at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD32 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.FC
Flow cytometric analysis of K-562 cells labeling CD32. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
CD32 was immunoprecipitated from 0.2 mg THP-1 cell lysate with abbit anti-CD32 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using abbit anti-CD32 antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input), Lane 2: abbit anti-CD32 antibody IP in THP-1 cell lysate, Lane 3: Rabbit IgG instead of abbit anti-CD32 antibody in THP-1 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds.| Product Name | CD32 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human CD32 aa 23-274 / 310. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:1000-1:5000 IHC:1:400 ICC/IF:1:100 FC:1:1000 IP: 1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FCGR2A |
|---|---|
| Gene Synonyms | CD32 FCG2 FcGR CD32A CDw32 FCGR2 IGFR2 FCGR2A1 FcgammaRIIa |
| Gene Full Name | Fc gamma receptor IIa |
| Gene Summary | This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008] |
| Alternative Names | CD32 antibody CD32A antibody CD32B antibody CD32C antibody CDw32 antibody Fc fragment of IgG low affinity IIa receptor antibody Fc fragment of IgG low affinity IIa receptor for (CD32) antibody Fc fragment of IgG low affinity IIb receptor antibody Fc fragment of IgG low affinity IIb receptor for (CD32) antibody Fc fragment of IgG low affinity IIc receptor antibody CD32 antibody CD32A antibody CD32B antibody CD32C antibody CDw32 antibody Fc fragment of IgG low affinity IIa receptor antibody Fc fragment of IgG low affinity IIa receptor for (CD32) antibody Fc fragment of IgG low affinity IIb receptor antibody Fc fragment of IgG low affinity IIb receptor for (CD32) antibody Fc fragment of IgG low affinity IIc receptor antibody Fc fragment of IgG low affinity IIc receptor for (CD32) antibody Fc fragment of IgG low affinity II receptor for (CD32) antibody Fc fragment of IgG low affinity IIa receptor (CD32) antibody Fc fragment of IgG low affinity IIb receptor (CD32) antibody Fc gamma receptor IIB antibody Fc gamma receptor IIC antibody Fc gamma RII a antibody Fc gamma RII b antibody Fc gamma RII c antibody Fc gamma RIIb antibody Fc gamma RIIc antibody Fc receptor IgG low affinity IIb antibody Fc-gamma RII-a antibody Fc-gamma RII-b antibody Fc-gamma RII-c antibody Fc-gamma-RIIa antibody FC-gamma-RIIB antibody Fc-GAMMA-RIIC antibody Fc[g]RII antibody FCG2 antibody FCG2A_HUMAN antibody FcGR antibody FCGR2 antibody FCGR2A antibody FCGR2A1 antibody FCGR2B antibody FCGR2C antibody Fcgr3 antibody Fcgr3a antibody FcgRII antibody Fcr-2 antibody Fcr-3 antibody FcRII a antibody FCRII antibody FcRII b antibody FcRII c antibody FcRII-a antibody FcRIIC antibody IGFR2 antibody IgG Fc receptor II a antibody IgG Fc receptor II b antibody IgG Fc receptor II beta antibody IgG Fc receptor II c antibody IgG Fc receptor II-a antibody IgG Fc receptor II-b antibody IgG Fc receptor II-c antibody Immunoglobulin G Fc receptor II antibody Immunoglobulin G Fc receptor II c antibody Low affinity immunoglobulin gamma Fc region receptor II antibody Low affinity immunoglobulin gamma Fc region receptor II-a antibody Low affinity immunoglobulin gamma Fc region receptor II-b antibody Low affinity immunoglobulin gamma Fc region receptor II-c antibody Ly-17 antibody Ly-m20 antibody LyM-1 antibody Lymphocyte antigen 17 antibody |
| Molecular Weight(MW) | 35 kDa(Observed band size: 40/31 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD32 on different lysates with Rabbit anti-CD32 antibody at 1/2,000 dilution. Lane 1: THP-1 cell lysate, Lane 2: THP-1 cell lysate treated with deglycosylation, Lane 3: K-562 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD32 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of K-562 cells labeling CD32 with Rabbit anti-CD32 antibody at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD32 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
FC
Flow cytometric analysis of K-562 cells labeling CD32. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
CD32 was immunoprecipitated from 0.2 mg THP-1 cell lysate with abbit anti-CD32 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using abbit anti-CD32 antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input), Lane 2: abbit anti-CD32 antibody IP in THP-1 cell lysate, Lane 3: Rabbit IgG instead of abbit anti-CD32 antibody in THP-1 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds.| Application Notes | WB:1:1000-1:5000 IHC:1:400 ICC/IF:1:100 FC:1:1000 IP: 1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
