WB
Western blot analysis of Phospho-ErbB3/HER3 (Y1289) on different lysates with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody at 1/1,000 dilution. Lane 1: MCF7 cell lysate, Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of MCF7 cells serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes labeling Phospho-ErbB3/HER3 (Y1289) with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | Phospho-ErbB3/HER3 (Y1289) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to a region surrounding Tyr1289 of Human ErbB3. |
| Modification | P-Y1289 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000 ICC/IF:1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ERBB3 |
|---|---|
| Gene Synonyms | HER3 FERLK LCCS2 VSCN1 ErbB-3 c-erbB3 erbB3-S MDA-BF-1 c-erbB-3 p180-ErbB3 p45-sErbB3 p85-sErbB3 |
| Gene Full Name | erb-b2 receptor tyrosine kinase 3 |
| Gene Summary | This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008] |
| Alternative Names | c erbB 3 antibody c erbB3 antibody Erb b2 receptor tyrosine kinase 3 antibody ErbB 3 antibody ERBB3 antibody ERBB3 protein antibody erbB3 S antibody ERBB3_HUMAN antibody Glial growth factor receptor antibody HER 3 antibody c erbB 3 antibody c erbB3 antibody Erb b2 receptor tyrosine kinase 3 antibody ErbB 3 antibody ERBB3 antibody ERBB3 protein antibody erbB3 S antibody ERBB3_HUMAN antibody Glial growth factor receptor antibody HER 3 antibody HER3 antibody Human epidermal growth factor receptor 3 antibody LCCS2 antibody MDA BF 1 antibody MGC88033 antibody p180 ErbB3 antibody p45 sErbB3 antibody p85 sErbB3 antibody proto-oncogene-like protein c ErbB 3 antibody proto-oncogene-like protein c ErbB3 antibody Proto-oncogene-like protein c-ErbB-3 antibody Receptor tyrosine protein kinase erbB 3 antibody Receptor tyrosine protein kinase erbB3 antibody Receptor tyrosine-protein kinase erbB-3 antibody Tyrosine kinase type cell surface receptor HER3 antibody Tyrosine kinase-type cell surface receptor HER3 antibody v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody |
| Molecular Weight(MW) | 148kDa(Observed band size: 180kDa) |
| Cellular Localization | Cell membrane, secreted. |
WB
Western blot analysis of Phospho-ErbB3/HER3 (Y1289) on different lysates with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody at 1/1,000 dilution. Lane 1: MCF7 cell lysate, Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of MCF7 cells serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes labeling Phospho-ErbB3/HER3 (Y1289) with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
