WB
Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody at 1/2000 dilution. Lane 1: A20 cell lysate, Lane 2: NIH/3T3 cell lysate (negative), Lane 3: MEF cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-p16INK4a antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | p16INK4a Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:200 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Cdkn2a |
|---|---|
| Gene Synonyms | Arf p16 MTS1 Pctr1 p19ARF p16INK4a p19 ARF-INK4a INK4a-ARF Ink4a/Arf p16(INK4a) |
| Gene Full Name | cyclin dependent kinase inhibitor 2A |
| Gene Summary | Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; MDM2/MDM4 family protein binding activity; and enzyme inhibitor activity. Involved in several processes, including cellular response to gamma radiation; negative regulation of lymphocyte proliferation; and positive regulation of apoptotic process. Acts upstream of or within several processes, including keratinocyte proliferation; negative regulation of mammary gland epithelial cell proliferation; and negative regulation of protein metabolic process. Located in cytoplasm and granular component. Part of protein-containing complex. Is expressed in several structures, including central nervous system; eye; genitourinary system; immune system; and umbilical artery. Used to study high grade glioma; malignant peripheral nerve sheath tumor; melanoma (multiple); and pancreatic carcinoma (multiple). Human ortholog(s) of this gene implicated in several diseases, including breast cancer (multiple); carcinoma (multiple); hematologic cancer (multiple); high grade glioma (multiple); and melanoma (multiple). Orthologous to human CDKN2A (cyclin dependent kinase inhibitor 2A). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | CCM2 antibody CDK4 inhibitor p16 INK4 antibody CDK4I antibody CDKN2 antibody CDKN2A antibody Cell cycle negative regulator beta antibody CMM2 antibody Cyclin dependent kinase 4 inhibitor A antibody Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody Cyclin Dependent Kinase Inhibitor 2A antibody CCM2 antibody CDK4 inhibitor p16 INK4 antibody CDK4I antibody CDKN2 antibody CDKN2A antibody Cell cycle negative regulator beta antibody CMM2 antibody Cyclin dependent kinase 4 inhibitor A antibody Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody Cyclin Dependent Kinase Inhibitor 2A antibody Cyclin dependent kinase inhibitor 2A isoform 4 antibody Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody Cyclin dependent kinase inhibitor p16 antibody INK4 antibody INK4A antibody MLM antibody MTS1 antibody Multiple tumor suppressor 1 antibody p14 antibody p16 antibody P16INK4 antibody p16INK4a antibody p19 antibody p19Arf antibody TP16 antibody |
| Molecular Weight(MW) | 18kDa(Observed band size:16kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of p16INK4a on different lysates with Rabbit anti-p16INK4a antibody at 1/2000 dilution. Lane 1: A20 cell lysate, Lane 2: NIH/3T3 cell lysate (negative), Lane 3: MEF cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-p16INK4a antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:2000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
