WB
Western blot analysis of Eph receptor A2 on different lysates with Rabbit anti-Eph receptor A2 antibody at 1/2000 dilution. Lane 1: A549 cell lysate, Lane 2: HEK-293 cell lysate, Lane 3: HeLa cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of A549 cells labeling Eph receptor A2 with Rabbit anti-Eph receptor A2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor A2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of A549 cells labeling Eph receptor A2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
Eph receptor A2 was immunoprecipitated from 0.2 mg A549 cell lysate with Rabbit anti-Eph receptor A2 antibody at 2µg/10µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Eph receptor A2 antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1:A549 cell lysate (input), Lane 2:Rabbit anti-Eph receptor A2 antibody IP in A549 cell lysate, Lane 3:Rabbit IgG instead of Rabbit anti-Eph receptor A2 antibody in A549 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 59 seconds.| Product Name | Eph receptor A2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human EPHA2 aa 10-550 / 976 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIPWB |
| WB:1:2000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EPHA2 |
|---|---|
| Gene Synonyms | ECK CTPA ARCC2 CTPP1 CTRCT6 |
| Gene Full Name | EPH receptor A2 |
| Gene Summary | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010] |
| Alternative Names | ARCC2 antibody AW545284 antibody CTPA antibody CTPP1 antibody CTRCT6 antibody EC 2.7.10.1 antibody Eck antibody Eph receptor A2 antibody EPHA2 antibody EPHA2_HUMAN antibody ARCC2 antibody AW545284 antibody CTPA antibody CTPP1 antibody CTRCT6 antibody EC 2.7.10.1 antibody Eck antibody Eph receptor A2 antibody EPHA2 antibody EPHA2_HUMAN antibody Ephrin receptor antibody Ephrin receptor EphA2 antibody Ephrin type A receptor 2 antibody Ephrin type-A receptor 2 antibody Epithelial cell kinase antibody Epithelial cell receptor protein tyrosine kinase antibody Myk 2 antibody Myk2 antibody Sek 2 antibody Sek2 antibody Soluble EPHA2 variant 1 antibody Tyrosine protein kinase receptor ECK antibody Tyrosine-protein kinase receptor ECK antibody Tyrosine-protein kinase receptor MPK-5 antibody Tyrosine-protein kinase receptor SEK-2 antibody |
| Molecular Weight(MW) | 108kDa(Observed band size:130kDa) |
| Cellular Localization | Cell membrane, Cell projection, ruffle membrane, lamellipodium membrane, Cell junction, focal adhesion. |
WB
Western blot analysis of Eph receptor A2 on different lysates with Rabbit anti-Eph receptor A2 antibody at 1/2000 dilution. Lane 1: A549 cell lysate, Lane 2: HEK-293 cell lysate, Lane 3: HeLa cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of A549 cells labeling Eph receptor A2 with Rabbit anti-Eph receptor A2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor A2 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of A549 cells labeling Eph receptor A2. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
Eph receptor A2 was immunoprecipitated from 0.2 mg A549 cell lysate with Rabbit anti-Eph receptor A2 antibody at 2µg/10µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-Eph receptor A2 antibody at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1:A549 cell lysate (input), Lane 2:Rabbit anti-Eph receptor A2 antibody IP in A549 cell lysate, Lane 3:Rabbit IgG instead of Rabbit anti-Eph receptor A2 antibody in A549 cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 59 seconds.| Application Notes | WB:1:2000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
