WB
Western blot analysis of Lck on different lysates with Rabbit anti-Lck antibody at 1/2000 dilution. Lane 1: Ramos cell lysate (20 µg/Lane), Lane 2: Raji cell lysate (20 µg/Lane), Lane 3: HeLa cell lysate (negative) (20 µg/Lane), Lane 4: Jurkat cell lysate (20 µg/Lane), Lane 5: MOLT-4 cell lysate (20 µg/Lane), Lane 6: Mouse spleen tissue lysate (40 µg/Lane), Lane 7: Rat spleen tissue lysate (40 µg/Lane), Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling Lck with Rabbit anti-Lck antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lck antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L ( 594) was used as the secondary antibody at 1/1,000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Lck antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Lck antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of HeLa (left, negative) and Jurkat (right, positive) cells labeling Lck. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
Lck was immunoprecipitated from 0.2 mg Jurkat cell lysate with Rabbit anti-Lck antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-Lck antibody at 1/1000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody Rabbit anti-Lck antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input), Lane 2: Rabbit anti-Lck antibody IP in Jurkat cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Lck antibody in Jurkat cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 4 seconds.| Product Name | Lck Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant protein within human Lck aa 1-150. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:2000 ICC/IF:1:500 IHC:1:1000 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Lck |
|---|---|
| Gene Synonyms | Lsk Lskt Hck-3 p56Lck p56 |
| Gene Full Name | lymphocyte protein tyrosine kinase |
| Gene Summary | Enables several functions, including CD4 receptor binding activity; protein antigen binding activity; and protein phosphatase binding activity. Involved in release of sequestered calcium ion into cytosol. Acts upstream of or within several processes, including positive regulation of macromolecule metabolic process; protein phosphorylation; and regulation of T cell differentiation. Located in cell-cell junction. Colocalizes with plasma membrane. Is expressed in several structures, including 2-cell stage embryo; nervous system; neural ectoderm; sensory organ; and thymus primordium. Human ortholog(s) of this gene implicated in immunodeficiency 22. Orthologous to human LCK (LCK proto-oncogene, Src family tyrosine kinase). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | IMD22 antibody LCK antibody Lck p56 antibody LCK proto-oncogene Src family tyrosine kinase antibody LCK_HUMAN antibody Leukocyte C-terminal Src kinase antibody LSK antibody Lymphocyte cell specific protein tyrosine kinase antibody Lymphocyte cell-specific protein-tyrosine kinase antibody Lymphocyte specific protein tyrosine kinase antibody IMD22 antibody LCK antibody Lck p56 antibody LCK proto-oncogene Src family tyrosine kinase antibody LCK_HUMAN antibody Leukocyte C-terminal Src kinase antibody LSK antibody Lymphocyte cell specific protein tyrosine kinase antibody Lymphocyte cell-specific protein-tyrosine kinase antibody Lymphocyte specific protein tyrosine kinase antibody Membrane associated protein tyrosine kinase antibody Oncogene lck antibody P56 LCK antibody p56(LSTRA) protein tyrosine kinase antibody p56-LCK antibody p56lck antibody pp58 lck antibody pp58lck antibody Protein YT16 antibody Proto oncogene tyrosine protein kinase LCK antibody Proto-oncogene Lck antibody Protooncogene tyrosine protein kinase LCK antibody T cell specific protein tyrosine kinase antibody T cell-specific protein-tyrosine kinase antibody T lymphocyte specific protein tyrosine kinase p56lck antibody Tyrosine-protein kinase Lck antibody YT 16 antibody YT16 antibody |
| Molecular Weight(MW) | 58kDa(Observed band size: 55kDa) |
| Cellular Localization | Cell membrane, Cytoplasm, cytosol. |
WB
Western blot analysis of Lck on different lysates with Rabbit anti-Lck antibody at 1/2000 dilution. Lane 1: Ramos cell lysate (20 µg/Lane), Lane 2: Raji cell lysate (20 µg/Lane), Lane 3: HeLa cell lysate (negative) (20 µg/Lane), Lane 4: Jurkat cell lysate (20 µg/Lane), Lane 5: MOLT-4 cell lysate (20 µg/Lane), Lane 6: Mouse spleen tissue lysate (40 µg/Lane), Lane 7: Rat spleen tissue lysate (40 µg/Lane), Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling Lck with Rabbit anti-Lck antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lck antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L ( 594) was used as the secondary antibody at 1/1,000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-Lck antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Lck antibody at 1/1000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of HeLa (left, negative) and Jurkat (right, positive) cells labeling Lck. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
Lck was immunoprecipitated from 0.2 mg Jurkat cell lysate with Rabbit anti-Lck antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-Lck antibody at 1/1000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody Rabbit anti-Lck antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input), Lane 2: Rabbit anti-Lck antibody IP in Jurkat cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-Lck antibody in Jurkat cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 4 seconds.| Application Notes | WB:1:2000 ICC/IF:1:500 IHC:1:1000 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
