WB
Western blot analysis of lysates from Mouse liver, using Rev-Erbα/NR1D1 Rabbit mAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB
Western blot analysis of various lysates using Rev-Erbα/NR1D1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB
Western blot analysis of lysates from HepG2 cells, using Rev-Erbα/NR1D1 Rabbit mAb at1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.ICC/IF
Confocal imaging of Hep G2 cells using RevErbα/NR1D1 Rabbit mAb (dilution1:200) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.ICC/IF
Confocal imaging of paraffin-embeddedMouse brain tissue using Rev-Erbα/NR1D1Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of Rat brain tissue usingRev-Erbα/NR1D1 Rabbit mAb (dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). Highpressure antigen retrieval performed with0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Product Name | Rev-Erbα/NR1D1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PWB |
| WB:1:1000-1:2000 ICC/IF:1:100-1:400 IF-P:1:100-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR1D1 |
|---|---|
| Gene Synonyms | EAR1 hRev THRA1 THRAL ear-1 REVERBA REVERBalpha |
| Gene Full Name | nuclear receptor subfamily 1 group D member 1 |
| Gene Summary | This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Jan 2013] |
| Alternative Names | EAR1 hRev THRA1 THRAL ear-1 REVERBA REVERBalpha Rev-Erbα/NR1D1 |
| Molecular Weight(MW) | 67kDa(Observed band size:75kDa) |
| Cellular Localization | cytoplasm, dendrite, dendritic spine, nuclear body, nucleoplasm, nucleus. |
WB
Western blot analysis of lysates from Mouse liver, using Rev-Erbα/NR1D1 Rabbit mAb at 1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
WB
Western blot analysis of various lysates using Rev-Erbα/NR1D1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
WB
Western blot analysis of lysates from HepG2 cells, using Rev-Erbα/NR1D1 Rabbit mAb at1:1000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
ICC/IF
Confocal imaging of Hep G2 cells using RevErbα/NR1D1 Rabbit mAb (dilution1:200) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of paraffin-embeddedMouse brain tissue using Rev-Erbα/NR1D1Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of Rat brain tissue usingRev-Erbα/NR1D1 Rabbit mAb (dilution 1:200) followed by a furtherincubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). Highpressure antigen retrieval performed with0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:100-1:400 IF-P:1:100-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
