WB
Western blot analysis of lysates from wild type (WT) and Smad3 knockout (KO) 293T cells, using [KOValidated] Smad3 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB
Western blot analysis of lysates from A-431 cells, using [KO Validated] Smad3 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffinembedded Human liver using [KO Validated]Smad3 Rabbit mAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.IP
Immunoprecipitation analysis of 300 μg extracts of A-431 cells using 3 μg Smad3 antibody.Western blot was performed from the immunoprecipitate using Smad3 antibody at a dilution of 1:500.| Product Name | [KO Validated] Smad3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:4000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD3 |
|---|---|
| Gene Synonyms | LDS3 mad3 LDS1C MADH3 JV15-2 hMAD-3 hSMAD3 HSPC193 HsT17436 |
| Gene Full Name | SMAD family member 3 |
| Gene Summary | The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3. [provided by RefSeq, May 2022] |
| Alternative Names | LDS3 mad3 LDS1C MADH3 JV15-2 hMAD-3 hSMAD3 HSPC193 HsT17436 d3 |
| Molecular Weight(MW) | 48kDa(Observed band size:52kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from wild type (WT) and Smad3 knockout (KO) 293T cells, using [KOValidated] Smad3 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
WB
Western blot analysis of lysates from A-431 cells, using [KO Validated] Smad3 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver using [KO Validated]Smad3 Rabbit mAb at dilution of1:100 (40x lens). Microwave antigen retrievalperformed with 0.01M PBS Buffer (pH 7.2)prior to IHC staining.
IP
Immunoprecipitation analysis of 300 μg extracts of A-431 cells using 3 μg Smad3 antibody.Western blot was performed from the immunoprecipitate using Smad3 antibody at a dilution of 1:500.| Application Notes | WB:1:1000-1:4000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
