WB
Western blot analysis of various lysates, using PGAM5 Rabbit mAb at1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.ICC/IF
Confocal imaging of Hep G2 cells usingPGAM5 Rabbit mAb (dilution1:300)(Red). The cells were counterstainedwith α-Tubulin Mouse mAb (dilution1:400) (Green). DAPI was used for nuclearstaining (blue). Objective: 60x.IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue usingPGAM5 Rabbit mAb at a dilution of1:500 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.| Product Name | PGAM5 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:20000-1:80000 ICC/IF:1:300-1:1200 IHC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PGAM5 |
|---|---|
| Gene Synonyms | BXLBV68 |
| Gene Full Name | PGAM family member 5, mitochondrial serine/threonine protein phosphatase |
| Gene Summary | Enables GTPase activator activity and protein serine/threonine phosphatase activity. Involved in necroptotic process. Located in mitochondrial inner membrane. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | BXLBV68 PGAM5 |
| Molecular Weight(MW) | 32/28kDa(Observed band size:28/32kDa) |
| Cellular Localization | mitochondrial outer membrane, mitochondrion. |
WB
Western blot analysis of various lysates, using PGAM5 Rabbit mAb at1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
ICC/IF
Confocal imaging of Hep G2 cells usingPGAM5 Rabbit mAb (dilution1:300)(Red). The cells were counterstainedwith α-Tubulin Mouse mAb (dilution1:400) (Green). DAPI was used for nuclearstaining (blue). Objective: 60x.
IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer tissue usingPGAM5 Rabbit mAb at a dilution of1:500 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer (pH 9.0) prior to IHC staining.| Application Notes | WB:1:20000-1:80000 ICC/IF:1:300-1:1200 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
