WB
Western blot analysis of various lysates, using PGAM5 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.ICC/IF
Immunofluorescence analysis of MCF7 cellsusing PGAM5 Rabbit pAb at dilutionof 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingPGAM5 Rabbit pAb at dilution of1:20 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.| Product Name | PGAM5 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PGAM5 |
|---|---|
| Gene Synonyms | BXLBV68 |
| Gene Full Name | PGAM family member 5, mitochondrial serine/threonine protein phosphatase |
| Gene Summary | Enables GTPase activator activity and protein serine/threonine phosphatase activity. Involved in necroptotic process. Located in mitochondrial inner membrane. [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | BXLBV68 PGAM5 |
| Molecular Weight(MW) | 32kDa(Observed band size:28/30kDa) |
| Cellular Localization | mitochondrial outer membrane, mitochondrion. |
WB
Western blot analysis of various lysates, using PGAM5 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of MCF7 cellsusing PGAM5 Rabbit pAb at dilutionof 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingPGAM5 Rabbit pAb at dilution of1:20 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
