WB
Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.IF-P
Immunofluorescence analysis of paraffinembedded rat ovary using FOXL2 Rabbit pAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.IF-P
Immunofluorescence analysis of paraffinembedded mouse ovary using FOXL2 RabbitpAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.| Product Name | FOXL2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PWB |
| WB:1:500-1:1000 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXL2 |
|---|---|
| Gene Synonyms | BPES PFRK POF3 BPES1 PINTO |
| Gene Full Name | forkhead box L2 |
| Gene Summary | This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Expansion of a polyalanine repeat region and other mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jul 2016] |
| Alternative Names | BPES PFRK POF3 BPES1 PINTO FOXL2 |
| Molecular Weight(MW) | 39kDa(Observed band size:50kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of various lysates using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
IF-P
Immunofluorescence analysis of paraffinembedded rat ovary using FOXL2 Rabbit pAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.
IF-P
Immunofluorescence analysis of paraffinembedded mouse ovary using FOXL2 RabbitpAb at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goatanti-Rabbit IgG (H+L) at 1:500dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
