WB
Western blot analysis of various lysates using ZIP14 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.IHC
Immunohistochemistry analysis of paraffinembedded Human liver cancer using ZIP14Rabbit pAb at dilution of 1:100(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat liver using ZIP14 Rabbit pAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHCstaining.| Product Name | ZIP14 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC39A14 |
|---|---|
| Gene Synonyms | HCIN NET34 ZIP14 cig19 HMNDYT2 LZT-Hs4 |
| Gene Full Name | solute carrier family 39 member 14 |
| Gene Summary | This gene encodes a member of the the SLC39A family of divalent metal transporters that mediates the cellular uptake of manganese, zinc, iron, and cadmium. The encoded protein contains eight transmembrane domains, a histidine-rich motif, and a metalloprotease motif, and is expressed on the plasma membrane and the endocytic vesicle membrane. It is an important transporter of nontransferrin-bound iron and a critical regulator of manganese homeostasis. Naturally occurring mutations in this gene are associated with neurodegeneration with brain iron accumulation and early-onset parkinsonism-dystonia with hypermanganesemia. [provided by RefSeq, May 2017] |
| Alternative Names | HCIN NET34 ZIP14 cig19 HMNDYT2 LZT-Hs4 |
| Molecular Weight(MW) | 54kDa(Observed band size:54kDa) |
| Cellular Localization | Cell membrane, Cell projection, Multi-pass membrane protein, lamellipodium. |
WB
Western blot analysis of various lysates using ZIP14 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver cancer using ZIP14Rabbit pAb at dilution of 1:100(40x lens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat liver using ZIP14 Rabbit pAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHCstaining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
