WB
Western blot analysis of various lysates using ENTPD1/CD39 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.ICC/IF
Immunofluorescence analysis of Raw264.7cells using ENTPD1/CD39 Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of THP-1 cellsusing ENTPD1/CD39 Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human liver using ENTPD1/CD39Rabbit pAb at dilution of 1:20 (40xlens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.IF-P
Immunofluorescence analysis of paraffinembedded human placenta usingENTPD1/CD39 Rabbit pAb at dilutionof 1:100 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.| Product Name | ENTPD1/CD39 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENTPD1 |
|---|---|
| Gene Synonyms | CD39 SPG64 ATP-DPH ATPDase NTPDase-1 |
| Gene Full Name | ectonucleoside triphosphate diphosphohydrolase 1 |
| Gene Summary | The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] |
| Alternative Names | CD39 SPG64 ATPDase NTPDase-1 ENTPD1/CD39 |
| Molecular Weight(MW) | 58kDa(Observed band size:80kDa) |
| Cellular Localization | Membrane, Multi-pass membrane protein. |
WB
Western blot analysis of various lysates using ENTPD1/CD39 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
ICC/IF
Immunofluorescence analysis of Raw264.7cells using ENTPD1/CD39 Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of THP-1 cellsusing ENTPD1/CD39 Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver using ENTPD1/CD39Rabbit pAb at dilution of 1:20 (40xlens). High pressure antigen retrievalperformed with 0.01M Citrate buffer (pH 6.0)prior to IHC staining.
IF-P
Immunofluorescence analysis of paraffinembedded human placenta usingENTPD1/CD39 Rabbit pAb at dilutionof 1:100 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
