WB
Western blot analysis of lysates from Rat lung, using ICAM1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffinembedded Mouse lung using ICAM1 RabbitpAb at dilution of 1:100 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior toIHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat lung using ICAM1 Rabbit pAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Product Name | ICAM1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Icam1 |
|---|---|
| Gene Synonyms | CD54 Ly-47 Icam-1 MALA-2 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Gene Summary | This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response. [provided by RefSeq, Aug 2016] |
| Alternative Names | CD54 Ly-47 Icam-1 MALA-2 ICAM1 |
| Molecular Weight(MW) | 59kDa(Observed band size:85kDa) |
| Cellular Localization | cell surface, external side of plasma membrane, extracellular exosome, extracellular space, immunological synapse, plasma membrane. |
WB
Western blot analysis of lysates from Rat lung, using ICAM1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse lung using ICAM1 RabbitpAb at dilution of 1:100 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior toIHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat lung using ICAM1 Rabbit pAb at dilution of 1:100 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
