WB
Western blot analysis of various lysates using cIAP2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.ICC/IF
Immunofluorescence analysis of A-549 cellsusing cIAP2 Rabbit pAb at dilution of1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of C6 cellsusing cIAP2 Rabbit pAb at dilution of1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen using cIAP2 RabbitpAb at dilution of 1:20 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Product Name | cIAP2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:500 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BIRC3 |
|---|---|
| Gene Synonyms | AIP1 API2 MIHC CIAP2 HAIP1 HIAP1 IAP-1 MALT2 RNF49 c-IAP2 |
| Gene Full Name | baculoviral IAP repeat containing 3 |
| Gene Summary | This gene encodes a member of the IAP family of proteins that inhibit apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. The encoded protein inhibits apoptosis induced by serum deprivation but does not affect apoptosis resulting from exposure to menadione, a potent inducer of free radicals. It contains 3 baculovirus IAP repeats and a ring finger domain. Transcript variants encoding the same isoform have been identified. [provided by RefSeq, Aug 2011] |
| Alternative Names | AIP1 API2 MIHC CIAP2 HAIP1 HIAP1 IAP-1 MALT2 RNF49 c-IAP2 cIAP2 |
| Molecular Weight(MW) | 68kDa(Observed band size:75kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of various lysates using cIAP2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
ICC/IF
Immunofluorescence analysis of A-549 cellsusing cIAP2 Rabbit pAb at dilution of1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of C6 cellsusing cIAP2 Rabbit pAb at dilution of1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen using cIAP2 RabbitpAb at dilution of 1:20 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:100-1:500 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH7.3. |
