WB
Western blot analysis of various lysates using LONP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.IHC
Immunohistochemistry analysis of paraffinembedded Rat kidney using LONP1 RabbitpAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse kidney using LONP1 RabbitpAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHCstaining.IP
Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg LONP1 antibody.Western blot was performed from the immunoprecipitate using LONP1 at a dilution of 1:1000.| Product Name | LONP1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LONP1 |
|---|---|
| Gene Synonyms | LON LONP PIM1 hLON LonHS CODASS PRSS15 |
| Gene Full Name | lon peptidase 1, mitochondrial |
| Gene Summary | This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013] |
| Alternative Names | LON LONP PIM1 hLON LonHS CODASS PRSS15 LONP1 |
| Molecular Weight(MW) | 106kDa(Observed band size:106kDa) |
| Cellular Localization | Mitochondrion matrix. |
WB
Western blot analysis of various lysates using LONP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat kidney using LONP1 RabbitpAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse kidney using LONP1 RabbitpAb at dilution of 1:100 (40x lens).Microwave antigen retrieval performed with0.01M Tris/EDTA Buffer (pH 9.0) prior to IHCstaining.
IP
Immunoprecipitation analysis of 300 μg extracts of A-549 cells using 3 μg LONP1 antibody.Western blot was performed from the immunoprecipitate using LONP1 at a dilution of 1:1000.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
