WB
Western blot analysis of various lysates using ANPEP/CD13 Rabbit mAb at 1:17000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): 293TExposure time: 30s.IHC
Immunohistochemistry analysis of paraffinembedded Human kidney tissue usingANPEP/CD13 Rabbit mAb at adilution of 1:9000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse kidney tissue usingANPEP/CD13 Rabbit mAb at adilution of 1:9000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat kidney tissue usingANPEP/CD13 Rabbit mAb at adilution of 1:9000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedMouse kidney tissue using ANPEP/CD13Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of paraffin-embeddedMouse spleen tissue using ANPEP/CD13Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.IF-P
Confocal imaging of paraffin-embedded Ratkidney tissue using ANPEP/CD13 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.IF-P
Confocal imaging of paraffin-embedded Ratspleen tissue using ANPEP/CD13 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40xIF-P
Confocal imaging of paraffin-embeddedHuman kidney tissue using ANPEP/CD13Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.| Product Name | ANPEP/CD13 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:17000-1:100000 IHC:1:1000-1:10000 IF-P:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Anpep |
|---|---|
| Gene Synonyms | Apn AP-M AP-N Cd13 P150 |
| Gene Full Name | alanyl aminopeptidase, membrane |
| Gene Summary | Enables metallopeptidase activity. Involved in angiotensin maturation. Is active in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; genitourinary system; liver; and sensory organ. Orthologous to human ANPEP (alanyl aminopeptidase, membrane). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Apn AP-M AP-N Cd13 P150 CD13 |
| Molecular Weight(MW) | 109kDa(Observed band size:160kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of various lysates using ANPEP/CD13 Rabbit mAb at 1:17000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): 293TExposure time: 30s.
IHC
Immunohistochemistry analysis of paraffinembedded Human kidney tissue usingANPEP/CD13 Rabbit mAb at adilution of 1:9000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse kidney tissue usingANPEP/CD13 Rabbit mAb at adilution of 1:9000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat kidney tissue usingANPEP/CD13 Rabbit mAb at adilution of 1:9000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedMouse kidney tissue using ANPEP/CD13Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embeddedMouse spleen tissue using ANPEP/CD13Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embedded Ratkidney tissue using ANPEP/CD13 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x.
IF-P
Confocal imaging of paraffin-embedded Ratspleen tissue using ANPEP/CD13 Rabbit mAb(dilution 1:200) followed by afurther incubation with Cy3 Goat Anti-RabbitIgG (H+L) (dilution 1:500) (Red).DAPI was used for nuclear staining (Blue).High pressure antigen retrieval performedwith 0.01M Citrate Buffer (pH 6.0) prior to IFstaining. Objective: 40x
IF-P
Confocal imaging of paraffin-embeddedHuman kidney tissue using ANPEP/CD13Rabbit mAb (dilution 1:200)followed by a further incubation with Cy3Goat Anti-Rabbit IgG (H+L) (dilution1:500) (Red). DAPI was used for nuclearstaining (Blue). High pressure antigenretrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.| Application Notes | WB:1:17000-1:100000 IHC:1:1000-1:10000 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
