WB
Western blot analysis of various lysates, using SOCS1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.ICC/IF
Immunofluorescence analysis of PC-12 cellsusing SOCS1 Rabbit pAb at dilutionof 1:100 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingSOCS1 Rabbit pAb at dilution of1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse spleen using SOCS1 RabbitpAb at dilution of 1:200 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior toIHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat brain using SOCS1 Rabbit pAb at dilution of 1:200 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Product Name | SOCS1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOCS1 |
|---|---|
| Gene Synonyms | JAB CIS1 SSI1 TIP3 CISH1 SSI-1 TIP-3 AISIMD SOCS-1 |
| Gene Full Name | suppressor of cytokine signaling 1 |
| Gene Summary | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008] |
| Alternative Names | JAB CIS1 SSI1 TIP3 CISH1 SSI-1 TIP-3 AISIMD SOCS-1 SOCS1 |
| Molecular Weight(MW) | 24kDa(Observed band size:23kDa) |
| Cellular Localization | Cytoplasmic vesicle, Nucleus. |
WB
Western blot analysis of various lysates, using SOCS1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of PC-12 cellsusing SOCS1 Rabbit pAb at dilutionof 1:100 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingSOCS1 Rabbit pAb at dilution of1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse spleen using SOCS1 RabbitpAb at dilution of 1:200 (40x lens).High pressure antigen retrieval performedwith 0.01M Citrate buffer (pH 6.0) prior toIHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat brain using SOCS1 Rabbit pAb at dilution of 1:200 (40x lens). Highpressure antigen retrieval performed with0.01M Citrate buffer (pH 6.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
