WB
Western blot analysis of various lysates using POLRMT Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.ICC/IF
Immunofluorescence analysis of C6 cellsusing POLRMT Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.ICC/IF
Immunofluorescence analysis of U-2 OS cellsusing POLRMT Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IP
Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg POLRMT antibody.Western blot was performed from the immunoprecipitate using POLRMT antibody at a dilution of1:1000.| Product Name | POLRMT Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLRMT |
|---|---|
| Gene Synonyms | APOLMT MTRNAP MTRPOL COXPD55 h-mtRPOL |
| Gene Full Name | RNA polymerase mitochondrial |
| Gene Summary | This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq, Jul 2008] |
| Alternative Names | APOLMT MTRNAP MTRPOL COXPD55 h-mtRPOL POLRMT |
| Molecular Weight(MW) | 139kDa(Observed band size:139kDa) |
| Cellular Localization | Mitochondrion. |
WB
Western blot analysis of various lysates using POLRMT Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of C6 cellsusing POLRMT Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
ICC/IF
Immunofluorescence analysis of U-2 OS cellsusing POLRMT Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IP
Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg POLRMT antibody.Western blot was performed from the immunoprecipitate using POLRMT antibody at a dilution of1:1000.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
