WB
Western blot analysis of lysates from HeLa cells using Collagen IV Rabbit mAb at 1:7000 dilutionincubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10 s.WB
Western blot analysis of various lysates using Collagen IV Rabbit mAb at 1:7000 dilutionincubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45 s.WB
Western blot analysis of lysates from Mouse lung using Collagen IV Rabbit mAb at 1:7000dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45 s.DB
Dot-blot analysis of all sorts of peptidesusing Collagen IV Rabbit mAb antibody at 1:1000 dilution.| Product Name | Collagen IV Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBWB |
| WB:1:2000-1:8000 DB:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL4A4 |
|---|---|
| Gene Synonyms | BFH ATS2 BFH1 CA44 |
| Gene Full Name | collagen type IV alpha 4 chain |
| Gene Summary | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR. [provided by RefSeq, Jul 2008] |
| Alternative Names | BFH ATS2 BFH1 CA44 Collagen IV |
| Molecular Weight(MW) | 164kDa(Observed band size:164kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of lysates from HeLa cells using Collagen IV Rabbit mAb at 1:7000 dilutionincubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10 s.
WB
Western blot analysis of various lysates using Collagen IV Rabbit mAb at 1:7000 dilutionincubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45 s.
WB
Western blot analysis of lysates from Mouse lung using Collagen IV Rabbit mAb at 1:7000dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45 s.
DB
Dot-blot analysis of all sorts of peptidesusing Collagen IV Rabbit mAb antibody at 1:1000 dilution.| Application Notes | WB:1:2000-1:8000 DB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
