WB
Western blot analysis of lysates from HeLa cells using TAZ Rabbit PolymAb® at 1:3000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.WB
Western blot analysis of lysates from Mouse lung using TAZ Rabbit PolymAb® at 1:3000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30 s.IHC
Immunohistochemistry analysis of paraffinembedded Rat lung tissue using TAZ RabbitPolymAb® at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-ED.IHC
Immunohistochemistry analysis of paraffinembedded Mouse lung tissue using TAZRabbit PolymAb®at a dilutionof 1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer(pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human pancreas tissue using TAZRabbit PolymAb® at a dilutionof 1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer(pH 9.0) prior to IHC staining.IP
Immunoprecipitation of TAZ from 300 µg extracts of HeLa cells was performed using 0.5 µg of TAZ RabbitPolymAb®. Rabbit IgG isotype control was used to precipitate the Control IgGsample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of thetotal input. Western blot analysis of immunoprecipitates was conducted using TAZ Rabbit PolymAb® at a dilution of 1:1000.| Product Name | TAZ Rabbit PolymAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1500-1:9000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WWTR1 |
|---|---|
| Gene Synonyms | TAZ |
| Gene Full Name | WW domain containing transcription regulator 1 |
| Gene Summary | Enables transcription coactivator activity. Involved in several processes, including intracellular signal transduction; negative regulation of canonical Wnt signaling pathway; and positive regulation of cell differentiation. Located in cytosol and nuclear body. Is active in nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | TAZ |
| Molecular Weight(MW) | 44kDa(Observed band size:60/50-55kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from HeLa cells using TAZ Rabbit PolymAb® at 1:3000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.
WB
Western blot analysis of lysates from Mouse lung using TAZ Rabbit PolymAb® at 1:3000dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30 s.
IHC
Immunohistochemistry analysis of paraffinembedded Rat lung tissue using TAZ RabbitPolymAb® at a dilution of 1:200(40x lens). High pressure antigen retrievalperformed with 0.01M Tris-ED.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse lung tissue using TAZRabbit PolymAb®at a dilutionof 1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer(pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human pancreas tissue using TAZRabbit PolymAb® at a dilutionof 1:200 (40x lens). High pressure antigenretrieval performed with 0.01M Tris-EDTABuffer(pH 9.0) prior to IHC staining.
IP
Immunoprecipitation of TAZ from 300 µg extracts of HeLa cells was performed using 0.5 µg of TAZ RabbitPolymAb®. Rabbit IgG isotype control was used to precipitate the Control IgGsample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of thetotal input. Western blot analysis of immunoprecipitates was conducted using TAZ Rabbit PolymAb® at a dilution of 1:1000.| Application Notes | WB:1:1500-1:9000 IHC:1:50-1:200 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
