WB
Western blot analysis of various lysates using FSTL1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.ICC/IF
Immunofluorescence analysis of C6 cellsusing FSTL1 Rabbit pAb at dilutionof 1:100. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3cells using FSTL1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.ICC/IF
Immunofluorescence analysis of Humanplacenta using FSTL1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.| Product Name | FSTL1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FSTL1 |
|---|---|
| Gene Synonyms | FRP FSL1 OCC1 OCC-1 tsc36 |
| Gene Full Name | follistatin like 1 |
| Gene Summary | This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008] |
| Alternative Names | FRP FSL1 OCC1 OCC-1 tsc36 MIR198 FSTL1 |
| Molecular Weight(MW) | 35kDa(Observed band size:45kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of various lysates using FSTL1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
ICC/IF
Immunofluorescence analysis of C6 cellsusing FSTL1 Rabbit pAb at dilutionof 1:100. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3cells using FSTL1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.
ICC/IF
Immunofluorescence analysis of Humanplacenta using FSTL1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclearstaining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
