WB
Western blot analysis of various lysates using RPA32/RPA2 Rabbit mAb at 1:25000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.ICC/IF
Confocal imaging of U-2 OS cells usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(AC012, dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.ICC/IF
Confocal imaging of HeLa cells usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.ICC/IF
Confocal imaging of NIH/3T3 cells usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.ICC/IF
Confocal imaging of C6 cells usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab ( dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing RPA32/RPA2 Rabbit mAb at adilution of 1:20000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer(pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse colon tissue usingRPA32/RPA2 Rabbit mAb at adilution of 1:20000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer(pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen tissue usingRPA32/RPA2 Rabbit mAb at adilution of 1:20000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer(pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embeddedHuman colon cancer tissue usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used fornuclear staining (Blue). High pressureantigen retrieval performed with 0.01MCitrate Buffer (pH 6.0) prior to IF staining.Objective: 40x.| Product Name | RPA32/RPA2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:15000-1:60000 ICC/IF:1:1000-1:4000 IHC:1:10000-1:40000 IF-P:1:1000-1:4000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPA2 |
|---|---|
| Gene Synonyms | REPA2 RPA32 RP-A p32 RP-A p34 |
| Gene Full Name | replication protein A2 |
| Gene Summary | This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017] |
| Alternative Names | REPA2 RPA32 RP-A p32 RP-A p34 |
| Molecular Weight(MW) | 29kDa(Observed band size:32kDa) |
| Cellular Localization | Nucleus, PML body. |
WB
Western blot analysis of various lysates using RPA32/RPA2 Rabbit mAb at 1:25000 dilutionincubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
ICC/IF
Confocal imaging of U-2 OS cells usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(AC012, dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of HeLa cells usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of NIH/3T3 cells usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab (dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of C6 cells usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells werecounterstained with α-Tubulin Mouse mAb(dilution 1:400) followed byincubation with ABflo® 488-conjugated GoatAnti-Mouse IgG (H+L) Ab ( dilution1:500) (Green). DAPI was used for nuclearstaining (Blue). Objective: 100x.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma tissueusing RPA32/RPA2 Rabbit mAb at adilution of 1:20000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer(pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse colon tissue usingRPA32/RPA2 Rabbit mAb at adilution of 1:20000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer(pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat spleen tissue usingRPA32/RPA2 Rabbit mAb at adilution of 1:20000 (40x lens). High pressureantigen retrieval performed with 0.01M TrisEDTA Buffer(pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embeddedHuman colon cancer tissue usingRPA32/RPA2 Rabbit mAb (dilution1:2000) followed by a further incubation withCy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used fornuclear staining (Blue). High pressureantigen retrieval performed with 0.01MCitrate Buffer (pH 6.0) prior to IF staining.Objective: 40x.| Application Notes | WB:1:15000-1:60000 ICC/IF:1:1000-1:4000 IHC:1:10000-1:40000 IF-P:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
