WB
Western blot analysis of lysates from wild type (WT) and 293T transfected with CD355/CRTAM, usingCD355/CRTAM Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution..Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30 s.WB
Western blot analysis of lysates from Human PBMC using CD355/CRTAM Rabbit mAb at 1:1000dilution incubated at room temperature for 1.5 hours. PBMC were treated with PMA (40 nM) andionomycin (2 nM) for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90 s.ICC/IF
Confocal imaging of 293F cells transfectedwith CD355/CRTAM using CD355/CRTAMRabbit mAb (dilution 1:200) followedby incubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). DAPIwas used for nuclear staining (blue).Objective: 100x.FC
Flow cytometry: 1X10^6 293T cells(negative control,left) and 293T(Transfection,right) cells were surfacestained with CD355/CRTAM Rabbit mAb(2 μg/mL,orange line) or ABflo® 647Rabbit IgG isotype control (5μl/Test,blue line), followed by Alexa Fluor®647 conjugated goat anti-rabbit pAb staining.Non-fluorescently stained cells were used asblank control (red line).FC
Flow cytometry: 1X10^6 293T(Transfection)cells were surface-stained with ABflo® 647Rabbit IgG isotype control (5μl/Test,left) or CD355/CRTAM RabbitmAb(2 μg/mL,right).| Product Name | CD355/CRTAM Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000-1:4000 ICC/IF:1:200-1:400 FC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRTAM |
|---|---|
| Gene Synonyms | CD355 |
| Gene Full Name | cytotoxic and regulatory T cell molecule |
| Gene Summary | The CRTAM gene is upregulated in CD4 (see MIM 186940)-positive and CD8 (see CD8A; MIM 186910)-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains (Yeh et al., 2008 [PubMed 18329370]).[supplied by OMIM, Feb 2009] |
| Alternative Names | CD355 CD355/CRTAM |
| Molecular Weight(MW) | 22/44kDa(Observed band size:66/80kDa) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
WB
Western blot analysis of lysates from wild type (WT) and 293T transfected with CD355/CRTAM, usingCD355/CRTAM Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution..Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30 s.
WB
Western blot analysis of lysates from Human PBMC using CD355/CRTAM Rabbit mAb at 1:1000dilution incubated at room temperature for 1.5 hours. PBMC were treated with PMA (40 nM) andionomycin (2 nM) for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90 s.
ICC/IF
Confocal imaging of 293F cells transfectedwith CD355/CRTAM using CD355/CRTAMRabbit mAb (dilution 1:200) followedby incubation with Cy3 Goat Anti-Rabbit IgG(H+L) (dilution 1:500) (Red). DAPIwas used for nuclear staining (blue).Objective: 100x.
FC
Flow cytometry: 1X10^6 293T cells(negative control,left) and 293T(Transfection,right) cells were surfacestained with CD355/CRTAM Rabbit mAb(2 μg/mL,orange line) or ABflo® 647Rabbit IgG isotype control (5μl/Test,blue line), followed by Alexa Fluor®647 conjugated goat anti-rabbit pAb staining.Non-fluorescently stained cells were used asblank control (red line).
FC
Flow cytometry: 1X10^6 293T(Transfection)cells were surface-stained with ABflo® 647Rabbit IgG isotype control (5μl/Test,left) or CD355/CRTAM RabbitmAb(2 μg/mL,right).| Application Notes | WB:1:1000-1:4000 ICC/IF:1:200-1:400 FC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
