WB
Western blot analysis of various lysates using TNFRSF17 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.WB
Western blot analysis of lysates from Rat spleen, using TNFRSF17 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.ICC/IF
Immunofluorescence analysis of SP2/0 cellsusing TNFRSF17 Rabbit pAb atdilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of U-937 cellsusing TNFRSF17 Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.| Product Name | TNFRSF17 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:100-1:500 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF17 |
|---|---|
| Gene Synonyms | BCM BCMA CD269 TNFRSF13A |
| Gene Full Name | TNF receptor superfamily member 17 |
| Gene Summary | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq, Jul 2008] |
| Alternative Names | BCM BCMA CD269 TNFRSF13A TNFRSF17 |
| Molecular Weight(MW) | 20kDa(Observed band size:15-35kDa) |
| Cellular Localization | Cell membrane, Endomembrane system, Single-pass type III membrane protein. |
WB
Western blot analysis of various lysates using TNFRSF17 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
WB
Western blot analysis of lysates from Rat spleen, using TNFRSF17 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.
ICC/IF
Immunofluorescence analysis of SP2/0 cellsusing TNFRSF17 Rabbit pAb atdilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of U-937 cellsusing TNFRSF17 Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.| Application Notes | WB:1:100-1:500 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
