ICC/IF
ICC staining Histone H2B(acetyl K20) (1/500) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining Histone H2B(acetyl K20) (1/50) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.ICC/IF
ICC staining Histone H2B(acetyl K20) (1/200) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H2B (acetyl K20) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.| Product Name | Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human Histone H2B aa 1-49/126. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHC |
| IHC:1:100-1:500 ICC/IF:1:100-1:500 ChIP:Use 0.5~2 μg for 25 μg of chromatin. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2BC12 |
|---|---|
| Gene Synonyms | H2BK H2B/S H2BFT H2BFAiii HIST1H2BK |
| Gene Full Name | H2B clustered histone 12 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family. The protein encoded is an antimicrobial protein with antibacterial and antifungal activity. Two transcripts that encode the same protein have been identified for this gene, which is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015] |
| Alternative Names | H2B 1A antibody H2B antibody H2B histone family antibody H2B2f antibody H2B2F_HUMAN antibody H2Ba antibody H2Bf antibody HIST2H2BF antibody histone H2B antibody histone H2B type 1 antibody H2B 1A antibody H2B antibody H2B histone family antibody H2B2f antibody H2B2F_HUMAN antibody H2Ba antibody H2Bf antibody HIST2H2BF antibody histone H2B antibody histone H2B type 1 antibody Histone H2B type 2-F antibody MGC131639 antibody H2BK20ac antibody |
| Cellular Localization | Chromosome, Nucleosome core, Nucleus. |
ICC/IF
ICC staining Histone H2B(acetyl K20) (1/500) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining Histone H2B(acetyl K20) (1/50) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC/IF
ICC staining Histone H2B(acetyl K20) (1/200) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody at 1/50 dilution. Counter stained with hematoxylin.
ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H2B (acetyl K20) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.| Application Notes | IHC:1:100-1:500 ICC/IF:1:100-1:500 ChIP:Use 0.5~2 μg for 25 μg of chromatin. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
