WB
Western blot analysis of Phospho-CDK1 (T161) on different lysates with Rabbit anti-Phospho-CDK1 (T161) antibody at 1/2000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 3: Jurkat cell lysate, Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 5: NIH/3T3 cell lysate, Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 7: PC-12 cell lysate, Lane 8: PC-12 treated with UV for 1 hour cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-CDK1 (T161) with Rabbit anti-Phospho-CDK1 (T161) antibody at 1/2500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-CDK1 (T161) antibody at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L ( 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1000 dilution.IHC
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-Phospho-CDK1 (T161) antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-CDK1 (T161). Cells were fixed and permeabilized. Then stained with the primary antibody (1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).FC
Flow cytometric analysis of NIH/3T3 cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-CDK1 (T161). Cells were fixed and permeabilized. Then stained with the primary antibody (1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Phospho-CDK1 (T161) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Thr161 of CDK1. |
| Modification | P-T161 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000 IHC:1:200 ICC/IF:1:2500 FC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK1 |
|---|---|
| Gene Synonyms | CDC2 CDC28A P34CDC2 |
| Gene Full Name | cyclin dependent kinase 1 |
| Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2023] |
| Alternative Names | Cdc 2 antibody Cdc2 antibody CDC28A antibody CDK 1 antibody CDK1 antibody CDK1_HUMAN antibody CDKN1 antibody CELL CYCLE CONTROLLER CDC2 antibody Cell division control protein 2 antibody Cell division control protein 2 homolog antibody Cdc 2 antibody Cdc2 antibody CDC28A antibody CDK 1 antibody CDK1 antibody CDK1_HUMAN antibody CDKN1 antibody CELL CYCLE CONTROLLER CDC2 antibody Cell division control protein 2 antibody Cell division control protein 2 homolog antibody Cell division cycle 2 G1 to S and G2 to M antibody Cell division protein kinase 1 antibody Cell Divsion Cycle 2 Protein antibody Cyclin Dependent Kinase 1 antibody Cyclin-dependent kinase 1 antibody DKFZp686L20222 antibody MGC111195 antibody p34 Cdk1 antibody p34 protein kinase antibody P34CDC2 antibody |
| Molecular Weight(MW) | 34kDa(Observed band size:34kDa) |
| Cellular Localization | Nucleus, Mitochondrion, Cytoplasm, cytoskeleton, microtubule organizing center, spindle. |
WB
Western blot analysis of Phospho-CDK1 (T161) on different lysates with Rabbit anti-Phospho-CDK1 (T161) antibody at 1/2000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 3: Jurkat cell lysate, Lane 4: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 5: NIH/3T3 cell lysate, Lane 6: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 7: PC-12 cell lysate, Lane 8: PC-12 treated with UV for 1 hour cell lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of Jurkat cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-CDK1 (T161) with Rabbit anti-Phospho-CDK1 (T161) antibody at 1/2500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-CDK1 (T161) antibody at 1/2,500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L ( 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1000 dilution.
IHC
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-Phospho-CDK1 (T161) antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of HeLa cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-CDK1 (T161). Cells were fixed and permeabilized. Then stained with the primary antibody (1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
FC
Flow cytometric analysis of NIH/3T3 cells treated with or without 100nM Calyculin A for 30 minutes labeling Phospho-CDK1 (T161). Cells were fixed and permeabilized. Then stained with the primary antibody (1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:200 ICC/IF:1:2500 FC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
