WB
Western blot analysis of PI3 Kinase p110α on different lysates with Rabbit anti-PI3 Kinase p110α antibody at 1/1000 dilution. Lane 1: HepG2 cell lysate, Lane 2: Hela cell lysate, Lane 3: Jurkat cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: mouse brain tissue lysate（20 µg/Lane), Lane 6: mouse liver tissue lysate（20 µg/Lane）, Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining of PI3 Kinase p110α in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).ICC/IF
ICC staining of PI3 Kinase p110α in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).ICC/IF
ICC staining of PI3 Kinase p110α in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PI3 Kinase p110α antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PI3 Kinase p110α antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
PI3 Kinase p110α was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-PI3 Kinase p110α antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-PI3 Kinase p110α antibody at 1/1000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-PI3 Kinase p110α antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PI3 Kinase p110α antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 2 minutes.| Product Name | PI3 Kinase p110α Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human PIK3CA aa 1001-1,050 / 1068. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3CA |
|---|---|
| Gene Synonyms | HMH MCM CCM4 CWS5 MCAP PI3K CLAPO CLOVE MCMTC PI3K-alpha p110-alpha |
| Gene Full Name | phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha |
| Gene Summary | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016] |
| Alternative Names | 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody caPI3K antibody CLOVE antibody CWS5 antibody MCAP antibody MCM antibody MCMTC antibody MGC142161 antibody MGC142163 antibody 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha antibody 5-bisphosphate 3-kinase catalytic subunit alpha isoform antibody caPI3K antibody CLOVE antibody CWS5 antibody MCAP antibody MCM antibody MCMTC antibody MGC142161 antibody MGC142163 antibody p110 alpha antibody p110alpha antibody Phosphatidylinositol 3 kinase catalytic alpha polypeptide antibody Phosphatidylinositol 3 kinase catalytic 110 KD alpha antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform antibody Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha antibody Phosphatidylinositol-4 antibody Phosphoinositide 3 kinase catalytic alpha polypeptide antibody PI3 kinase p110 subunit alpha antibody PI3-kinase subunit alpha antibody PI3K antibody PI3K-alpha antibody PI3KC A antibody PIK3C A antibody Pik3ca antibody PK3CA antibody PK3CA_HUMAN antibody PtdIns 3 kinase p110 antibody PtdIns-3-kinase subunit alpha antibody PtdIns-3-kinase subunit p110-alpha antibody Serine/threonine protein kinase PIK3CA antibody |
| Molecular Weight(MW) | 124kDa(Observed band size:111kDa) |
| Cellular Localization | Cytosol, plasma membrane, cytoplasm, lamellipodium, membrane, phosphatidylinositol 3-kinase complex, phosphatidylinositol 3-kinase complex, class IA. |
WB
Western blot analysis of PI3 Kinase p110α on different lysates with Rabbit anti-PI3 Kinase p110α antibody at 1/1000 dilution. Lane 1: HepG2 cell lysate, Lane 2: Hela cell lysate, Lane 3: Jurkat cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: mouse brain tissue lysate（20 µg/Lane), Lane 6: mouse liver tissue lysate（20 µg/Lane）, Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining of PI3 Kinase p110α in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC/IF
ICC staining of PI3 Kinase p110α in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC/IF
ICC staining of PI3 Kinase p110α in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PI3 Kinase p110α antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PI3 Kinase p110α antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
PI3 Kinase p110α was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-PI3 Kinase p110α antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-PI3 Kinase p110α antibody at 1/1000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: Rabbit anti-PI3 Kinase p110α antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-PI3 Kinase p110α antibody in HeLa cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 2 minutes.| Application Notes | WB:1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
