WB
Western blot analysis of RAP1A + RAP1B on different lysates with Rabbit anti-RAP1A + RAP1B antibody at 1/1000 dilution. Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: HEK-293 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature.FC
Flow cytometric analysis of HEK-293 cells labeling RAP1A + RAP1B. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | RAP1A + RAP1B Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human RAP1A aa 93-142 / 184. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:1000-1:2000 FC:1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAP1A |
|---|---|
| Gene Synonyms | RAP1 C21KG G-22K KREV1 KREV-1 SMGP21 |
| Gene Full Name | RAP1A, member of RAS oncogene family |
| Gene Summary | This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Alternative Names | C21KG antibody G 22K antibody G-22K antibody GTP binding protein smg p21A antibody GTP-binding protein smg p21A antibody KREV 1 antibody KREV1 antibody OTTHUMP00000013741 antibody RAP 1A antibody RAP1 antibody C21KG antibody G 22K antibody G-22K antibody GTP binding protein smg p21A antibody GTP-binding protein smg p21A antibody KREV 1 antibody KREV1 antibody OTTHUMP00000013741 antibody RAP 1A antibody RAP1 antibody RAP1A antibody RAP1A member of RAS oncogene family antibody RAP1A_HUMAN antibody Ras related protein Krev 1 antibody Ras related protein Rap 1A antibody RAS related protein RAP1A antibody Ras-related protein Krev-1 antibody Ras-related protein Rap-1A antibody SMGP21 antibody GTP-binding protein smg p21B antibody K-REV antibody OK/SW-cl.11 antibody RAL1B antibody rap1b antibody RAP1B member of RAS oncogene family antibody RAP1B_HUMAN antibody Ras family small GTP binding protein RAP1B antibody RAS related protein RAP1B antibody Ras-related protein Rap-1b antibody Small GTP binding protein antibody |
| Molecular Weight(MW) | 20kDa(Observed band size:20kDa) |
| Cellular Localization | Cell membrane, Cytoplasm, Cytoplasm, perinuclear region, Cell junction, Early endosome; Cell membrane, Cytoplasm, cytosol, Cell junction. |
WB
Western blot analysis of RAP1A + RAP1B on different lysates with Rabbit anti-RAP1A + RAP1B antibody at 1/1000 dilution. Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: NIH/3T3 cell lysate, Lane 4: HEK-293 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200000 dilution was used for 1 hour at room temperature.
FC
Flow cytometric analysis of HEK-293 cells labeling RAP1A + RAP1B. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000-1:2000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
