WB
Western blot analysis of Rubicon / Baron on different lysates with Rabbit anti-Rubicon / Baron antibody at 1/2000 dilution. Lane 1: Raji cell lysate, Lane 2: A549 cell lysate, Lane 3: NCI-H1299 cell lysate, Lane 4: HeLa cell lysate, Lane 5: 293T cell lysate, Lane 6: COS-1 cell lysate, Lane 7: RAW264.7 cell lysate, Lane 8: NIH/3T3 cell lysate, Lane 9: C6 cell lysate, Lane 10: Mouse spleen tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1: 18 seconds; Lane 2-10: 1 minute 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Rubicon / Baron Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Rubicon aa 1-350. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUBCN |
|---|---|
| Gene Synonyms | SCAR15 RUBICON KIAA0226 |
| Gene Full Name | rubicon autophagy regulator |
| Gene Summary | The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014] |
| Alternative Names | Baron antibody Beclin 1 associated RUN domain containing protein antibody Beclin-1 associated RUN domain containing protein antibody hypothetical protein LOC9711 antibody Kiaa0226 antibody OTTHUMP00000208021 antibody OTTHUMP00000208023 antibody OTTHUMP00000208024 antibody RUBIC_HUMAN antibody Rubicon antibody Baron antibody Beclin 1 associated RUN domain containing protein antibody Beclin-1 associated RUN domain containing protein antibody hypothetical protein LOC9711 antibody Kiaa0226 antibody OTTHUMP00000208021 antibody OTTHUMP00000208023 antibody OTTHUMP00000208024 antibody RUBIC_HUMAN antibody Rubicon antibody RUN domain and cysteine rich domain containing Beclin 1 interacting protein antibody Run domain Beclin 1 interacting and cystein rich containing protein antibody Run domain Beclin-1 interacting and cysteine-rich containing protein antibody RUN domain protein as Beclin 1 interacting and cysteine rich containing antibody |
| Molecular Weight(MW) | 109kDa(Observed band size:109kDa) |
| Cellular Localization | Late endosome, Lysosome, Early endosome. |
WB
Western blot analysis of Rubicon / Baron on different lysates with Rabbit anti-Rubicon / Baron antibody at 1/2000 dilution. Lane 1: Raji cell lysate, Lane 2: A549 cell lysate, Lane 3: NCI-H1299 cell lysate, Lane 4: HeLa cell lysate, Lane 5: 293T cell lysate, Lane 6: COS-1 cell lysate, Lane 7: RAW264.7 cell lysate, Lane 8: NIH/3T3 cell lysate, Lane 9: C6 cell lysate, Lane 10: Mouse spleen tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1: 18 seconds; Lane 2-10: 1 minute 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
