WB
Western blot analysis of Phospho-PKR (T451) on different lysates with Rabbit anti-Phospho-PKR (T451) antibody at 1/1000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, Lane 3: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-PKR (T451) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide corresponding to residues surrounding Thr45 of human PKR protein. |
| Modification | P-T451 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK2 |
|---|---|
| Gene Synonyms | PKR PRKR PPP1R83 |
| Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 2 |
| Gene Summary | The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses. [provided by RefSeq, Jul 2021] |
| Alternative Names | Double stranded RNA activated protein kinase antibody E2AK2_HUMAN antibody eIF-2A protein kinase 2 antibody EIF2AK1 antibody EIF2AK2 antibody Eukaryotic translation initiation factor 2 alpha kinase 2 antibody Eukaryotic translation initiation factor 2-alpha kinase 2 antibody HGNC:9437 antibody Interferon induced double stranded RNA activated protein kinase antibody Double stranded RNA activated protein kinase antibody E2AK2_HUMAN antibody eIF-2A protein kinase 2 antibody EIF2AK1 antibody EIF2AK2 antibody Eukaryotic translation initiation factor 2 alpha kinase 2 antibody Eukaryotic translation initiation factor 2-alpha kinase 2 antibody HGNC:9437 antibody Interferon induced double stranded RNA activated protein kinase antibody Interferon inducible elF2 alpha kinase antibody Interferon inducible RNA dependent protein kinase antibody Interferon-induced double-stranded RNA-activated protein kinase antibody Interferon-inducible RNA-dependent protein kinase antibody MGC126524 antibody P1/eIF-2A protein kinase antibody P1/eIF2A protein kinase antibody p68 kinase antibody PKR antibody PPP1R83 antibody PRKR antibody Protein kinase interferon inducible double stranded RNA dependent antibody Protein kinase RNA activated antibody Protein kinase RNA-activated antibody Protein phosphatase 1 regulatory subunit 83 antibody Serine/threonine protein kinase TIK antibody Tyrosine protein kinase EIF2AK2 antibody |
| Molecular Weight(MW) | 62kDa(Observed band size:70kDa) |
| Cellular Localization | Cytoplasm, Nucleus, perinuclear region. |
WB
Western blot analysis of Phospho-PKR (T451) on different lysates with Rabbit anti-Phospho-PKR (T451) antibody at 1/1000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, Lane 3: HeLa treated with 10ng/mL IFN-α1 for 18 hours then 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
