WB
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/5000 dilution. Lane 1: Daudi cell lysate, Lane 2: Raji cell lysate, Lane 3: Ramos cell lysate, Lane 4: K-562 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/5000 dilution. Lane 1: Raji cell lysate (10 µg/Lane), Lane 2: Mouse lung tissue lysate (20 µg/Lane), Lane 3: Rat lung tissue lysate (20 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1000 dilution.| Product Name | CD86 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human CD86 aa 1-50 / 329. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:5000-1:20000 ICC/IF:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD86 |
|---|---|
| Gene Synonyms | B70 B7-2 B7.2 BU63 FUN-1 LAB72 CD28LG2 CD86 v6 |
| Gene Full Name | CD86 molecule |
| Gene Summary | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011] |
| Alternative Names | Activation B7-2 antigen 3 antibody Activation B7-2 antigen antibody B-lymphocyte activation antigen B7-2 2 antibody B-lymphocyte activation antigen B7-2 antibody B7 2 antibody B7 antibody B7-2 antibody B7.2 antibody B70 antibody B72 antigen antibody Activation B7-2 antigen 3 antibody Activation B7-2 antigen antibody B-lymphocyte activation antigen B7-2 2 antibody B-lymphocyte activation antigen B7-2 antibody B7 2 antibody B7 antibody B7-2 antibody B7.2 antibody B70 antibody B72 antigen antibody BU63 antibody CD28 antigen ligand 2 2 antibody CD28 antigen ligand 2 antibody Cd28l2 antibody CD28LG2 antibody CD86 antibody CD86 antigen (CD28 antigen ligand 2 B7-2 antigen) 1 2 antibody CD86 antigen (CD28 antigen ligand 2 B7-2 antigen) antibody CD86 antigen antibody CD86 molecule antibody CD86_HUMAN antibody CLS1 antibody CTLA-4 counter-receptor B7.2 2 3 antibody CTLA-4 counter-receptor B7.2 antibody Early T-cell costimulatory molecule 1 antibody ETC-1 antibody FUN 1 antibody FUN-1 antibody LAB72 antibody Ly-58 antibody Ly58 antibody MB7 antibody MB7-2 antibody Membrane glycoprotein antibody MGC34413 antibody T-lymphocyte activation antigen CD86 antibody TS/A-2 antibody |
| Molecular Weight(MW) | 38kDa(Observed band size:70kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/5000 dilution. Lane 1: Daudi cell lysate, Lane 2: Raji cell lysate, Lane 3: Ramos cell lysate, Lane 4: K-562 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody at 1/5000 dilution. Lane 1: Raji cell lysate (10 µg/Lane), Lane 2: Mouse lung tissue lysate (20 µg/Lane), Lane 3: Rat lung tissue lysate (20 µg/Lane), Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody at 1/500 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1000 dilution.| Application Notes | WB:1:5000-1:20000 ICC/IF:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
