WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Osteopontin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HepG2.IHC
Human liver was stained with anti-Osteopontin rabbit antibody.| Product Name | Osteopontin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1000 ICC/IF:1:200-1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SPP1 |
|---|---|
| Gene Synonyms | OPN BNSP BSPI ETA-1 |
| Gene Full Name | secreted phosphoprotein 1 |
| Gene Summary | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | SPP1 BNSP OPN PSEC0156 Osteopontin Bone sialoprotein 1 Nephropontin Secreted phosphoprotein 1 SPP-1 Urinary stone protein Uropontin |
| Molecular Weight(MW) | 35kDa(Observed MW 55,65kDa) |
| Cellular Localization | Secreted |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Osteopontin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HepG2.
IHC
Human liver was stained with anti-Osteopontin rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1000 ICC/IF:1:200-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
